SB Carlsberg Flask

Breweries can now control aseptic yeast with the Alfa Laval SB Carlsberg Flask. Breweries, as well as the dairy, food and beverage industries use it to sterilise wort and grow pure yeast cultures on a scientific scale for yeast multiplication plants. The materials used to construct the flask are easily autoclavable and meet high sanitary requirements.

Alfa Laval SB is one possibility. There are numerous ways to optimise yeast management with Carlsberg Flask. These include a two-wheel trolley, various breathing and aeration filters and silicone hoses.

SB Carlsberg Flask Application

The SB Carlsberg Flask is a designed specifically design for the brewing sector

Related Industries

Beverage Processing

Benefits

1. Single-use aseptic design ensures efficacy
2. Sanitary and clear design
3. Safe and sterile transfer
4. Easily relocate to the chosen spot
5. Strong design for yeast survival and wort sterilising

The Alfa Laval SB Carlsberg Flask is a rectangular vessel with a flat bottom and a top lid. It features a micro sample port for aseptically introducing pure yeast culture, a membrane sampling valve for aeration and product transfer and breathing filters. Hose clips must be properly fastened to all hose tails before the pure yeast culture can be moved from the Alfa Laval SB Carlsberg Flask to the yeast propagation procedure.

Sterilized wort (about 80% of the total volume) is added to the flask until it reaches its net capacity, which can be accomplished using an electric hotplate, gas burner or autoclave. To get the wort to the proper temperature for working, place the flask in the refrigerator or a cold room. The aeration lance connects to the membrane sampling valve, which aerates the cold wort.

Using a syringe, aseptically inject yeast culture into the membrane fitting or transfer dried yeast culture to the flask via the empty filter housing.

Keeping the breathing filter linked to the flask makes sterilization easier. Reattaching the breathing filter, if sterilized separately, is critical during the final phase of wort sterilization, while steam is still evaporating from the flask, to ensure that the filter connection is also sterilized. To transfer the dry yeast culture to the flask, just use the empty filter housing as a funnel.

The next stage is to introduce oxygen to the cold wort using the aeration lance and membrane sampling valve. Using sterile air is critical. For safety concerns, put the wort via a filter attached to the device prior to the membrane sampling valve.

Foaming can be avoided by ensuring adequate air supply and flow. To avoid clogging or harming the breathing filter, make sure no foam comes into contact with it.

The simplest method for determining the optimal air supply conditions and required aeration time (less than 10 minutes) is to do the test with the flask lid open. Note that you cannot use the yeast culture from these tests.

After the wort has been sufficiently oxygenated, a 150-200 mL syringe is used to aseptically insert the yeast culture through the membrane fitting. The empty filter housing can also be used to put dry yeast culture into the flask. If you need extra yeast, unscrew the cover and transfer the culture to a flask. On the other hand, extra caution and a sterile environment are required for this work.

At the “high kräussend” stage, in an aseptic environment with a sterile air supply to the breathing filter, transfer the pure yeast culture to the multiplication plant. When inoculating yeast propagation plants, a sterile air supply of 2 bar is recommended, with a maximum pressure of 5 bar (72.5 psi) permitted under all conditions. After connecting the membrane sample valve on the Carlsberg flask lid to a sample device on the receiving vessel (the inoculator), the yeast culture can be moved from the flask to the inoculator.

Before transfer, disinfect all connections, including the connecting hose and membrane sample valve, with either alcohol or steam. The valve can be sealed with alcohol between operations and flushed with sterile air before to use.

We recommend that, once everything is connected and ready to inoculate, you open the sterile air supply, followed by the membrane sample valve atop the flask, and finally the sample device on the inoculator.

When the inoculator begins to bubble, it is best to leave the air supply on for a few minutes to ensure that the yeast culture is thoroughly transferred.

Finally, the receiving vessel’s sample device and the Alfa Laval SB Carlsberg Flask’s membrane sample valve can be closed to turn off the air supply.

After each use, disassemble the flask and hand-clean it with standard detergents.